- Grower Support Manager (Blueberries) - Harare, Zimbabwe
- Finance Director - Ukraine
- Sales Manager and Quality Assurance Supervisor
- Breeder (MSc) - Hann. Münden, Germany
- Buitendienst Medewerker - België
- Assistant Grower Manager Tomato & Capsicums - Malaysia Highlands
- Assistant Grower Manager Lettuce & Herbs - Malaysia Highlands
- Trainee Production Management - starting location Ethiopia
- Сhief technologist, Tula region - Russia
- Сhief technology officer, Tula region - Russia
Top 5 -yesterday
Top 5 -last week
Top 5 -last month
Producing transgenic virus B resistant chrysanthemum
Chrysanthemum (Chrysanthemum morifolium Ramat cv. White Snowdon) was transformed via Agrobacterium-mediated DNA delivery with the aim of improving resistance to CVB infection. Transformation vectors contain the nucleotide sequence of CVB coat proteins (CP) in sense, antisense, and double sense orientation.
The transformative vectors also invert repeats of CVB coat protein gene fragments for the induction of RNA-interference. The transgenic chrysanthemum plants were successfully obtained. The integration of the target sequences in plant genomes was confirmed by polymerase chain reaction (PCR) and Southern blot analyses.
Chrysanthemum lines were transformed with antisense, sense, and double sense CVB CP sequences, as well as with hairpin RNA-interference constructs that were assayed for resistance to CVB. Infection of transgenic plants by CVB through the grafting of infected scions shows resistance only among plants with carried double sense (16.7%) and hairpin (12.5%) constructs. The plants transformed by sense and double sense sequences were observed and classified as tolerant.
Access the full study at Agronomy
Publication date :
Receive the daily newsletter in your email for free | Click here
Other news in this sector:
- 2018-12-14 "Focal Scoop fulfills designers' needs"
- 2018-12-13 US: "Farm Bill will protect and encourage floriculture innovation"
- 2018-12-12 Colombia: New BallSB R&D facility opens its doors
- 2018-12-07 "I give people as much knowledge as possible"
- 2018-12-06 Brandkamp presents 2019 Chrysanthemum catalog
- 2018-12-03 Cut hydrangea for tropical growing
- 2018-12-03 New Zealand: Esplanade rose trials yield winning new blooms
- 2018-12-03 Nostalgic rose types for heated greenhouses
- 2018-11-30 How removing a trait can add value to a plant
- 2018-11-29 In praise of plant breeders
- 2018-11-29 Succulents on a stick
- 2018-11-28 "Breeding a good fragrant pot-rose is a whole new ball game"
- 2018-11-27 Agriom selects promising hibiscus varieties for South Europe
- 2018-11-27 Awards boost sales of Hydrangea Runaway Bride
- 2018-11-22 "Internode cuttings are more suited for the modern grower"
- 2018-11-21 "Sales Summer Breeze strawberry exceeded our expectations"
- 2018-11-21 Morel's seed production certified MPS
- 2018-11-21 "The story of a rose the public does not recognise as a rose"
- 2018-11-20 "Newest DNA technologies are going to bring major changes in floriculture"
- 2018-11-19 UK: Chrysanthemum breeder wins cup in memory of son