Recently, the Lily Research Group of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences has revealed the molecular mechanism of anthocyanin transport in two-color lily (‘Tiny Padhye’). Related research results were published online in "Genes".
Lily flowers are not only diverse in color, but also have rich variations in petal coloring types, and have important ornamental and commercial value. Anthocyanin is an important pigment for lily coloration. It is synthesized in the cytoplasm, but it needs to be transported into the vacuole to make the plant color. But so far, there are few reports on the transport mechanism of anthocyanins in lilies. The disclosure of the molecular mechanism of the anthocyanin transport in the two-color lily can provide a theoretical basis for the artificial regulation and molecular improvement of the flower color.
The research used RACE technology, bioinformatics analysis, subcellular localization, complementary experiments and other experimental methods to study the role of MATE transport protein in lily anthocyanin transport. Studies have shown that the protein encoded by the LhDTX35 gene in lily is an important transport membrane protein in the MATE gene family. Expression profiling data analysis showed that the LhDTX35 gene was positively correlated with the expression of anthocyanins in lily flowers of different developmental stages, and the gene was highly expressed in the colored parts of the lower petals and low in the colorless parts of the upper petals at S2. The complementary experiment of Arabidopsis mutants showed that LhDTX35 gene can restore the phenotype of Arabidopsis DTX35 mutant pods sterility and low anthocyanin content in hypocotyls. The experimental results showed that the Mate-like protein encoded by LhDTX35 gene in lily Involved in the transport of lily anthocyanins.
The paper takes the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences as the first unit to complete, Xu Hua, Ph.D. student of the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences is the first author and researcher Ming Jun and Assistant Researcher Xu Leifeng are the corresponding authors. The research was supported by the National Natural Science Foundation of China, the National Science and Technology Support Project, and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.