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Dutch consortium unravels first tulip genome
The tulip genome contains large chunks of identical DNA which occur extremely frequently. By isolating these chunks in an intact form and reading them with the help of a DNA sequencing technology, so that the ends have a unique DNA sequence, it is possible to map the entire genome with powerful bioinformatics tools.
The consortium has demonstrated this in recent months using sequencing technology from Oxford Nanopore Technologies and the TULIP algorithm (The Uncorrected Long-read Integration Process) developed by Generade and Future Genomics Technologies. A unique feature is that the genome of the very common garden tulip Tulipa gesneriana is the biggest genome that has ever been sequenced. Its size is estimated at approximately 34 giga base pairs, nearly 11 times larger than that of humans. Over the coming months, the DNA of the tulip variety Orange Sherpa will be repeatedly sequenced to give better resolution and the billions of pieces of the DNA puzzle will be fitted together with the help of TULIP.
The tulip genome reveals how the DNA determines the characteristics of a tulip. DNA patterns that are linked to desirable characteristics will soon be used in advanced breeding so that these characteristics can be combined to give new, improved varieties. With this knowledge, new tulip varieties which are resistant to common diseases can be developed faster and in a more targeted way. This will also reduce the use of plant protection products.
Hans van den Heuvel, Director Research & Development at Dümmen Orange: "The tulip genome makes the human genome look tiny: the entire human genome fits into one tulip chromosome. With the technology that was available at the turn of the century, this job would have taken over 100 years, an impossible task. But now we can do it in a matter of days. Data analysis is in full swing and there is no doubt that it will produce information which will transform tulip breeding, making it faster, more predictive and above all 'greener' because we will focus on varieties that can be grown sustainably."
Helma Kaptein, Director of Generade: "This genome will be made available in the public domain. It represents an important first step towards innovation in the tulip industry, a sector that is facing social and economic challenges, and will show the Netherlands, where the tulip is highly prized both culturally and as an icon, how public-private cooperation can achieve sustainable and innovative development in a traditional agricultural sector."
BaseClear is the biggest independent DNA analysis laboratory in the Netherlands. Bas Reichert, CEO of BaseClear, outlines his commitment to this project: "This project has demonstrated the impact and applicability of the latest DNA technologies in the ornamental horticulture sector. It proves that this technology is now feasible and affordable. The use of DNA technology will further accelerate developments in the ornamental sector and make them even more sustainable. We are proud that, with our partners in this project, we can make a contribution to a greener world in this way."
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