The work was funded by grants from the Biomaker 2017 and OpenPlant 2018 funding rounds and the work was carried out by Jennifer Deegan and Tim Deegan.
All of this experience came together recently when she entered the area of electronics and programming for biology in the 2017 Biomaker challenge. Her low budget, deep focus microscope design was created and built together with her software engineer husband Tim Deegan, and with help from the forum members at www.photomacrography.net. It worked well, and they went on to win the Biomaker spirit trophy.
Fern gametophytes are very beautiful structures, but are difficult to photograph, because of issues with depth of field in high magnification photography. The microscope (shown below) enables a camera to take many photographs of a gametophyte fern, while moving in tiny, micron scale, increments towards the subject. The focused areas of each photograph are then cut out and amalgamated into one single focused image.